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anova followed by the least significant degree (lsd) post hoc test  (GraphPad Software Inc)

 
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    GraphPad Software Inc anova followed by the least significant degree (lsd) post hoc test
    Anova Followed By The Least Significant Degree (Lsd) Post Hoc Test, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anova followed by the least significant degree (lsd) post hoc test/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
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    Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from <t>ANOVA</t> with Fisher’s least significant <t>difference</t> <t>(LSD)</t> post hoc test. *, p < 0.05; n.s., no statistical difference.
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    Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from <t>ANOVA</t> with Fisher’s least significant <t>difference</t> <t>(LSD)</t> post hoc test. *, p < 0.05; n.s., no statistical difference.
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    Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from <t>ANOVA</t> with Fisher’s least significant <t>difference</t> <t>(LSD)</t> post hoc test. *, p < 0.05; n.s., no statistical difference.
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    Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from <t>ANOVA</t> with Fisher’s least significant <t>difference</t> <t>(LSD)</t> post hoc test. *, p < 0.05; n.s., no statistical difference.
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    Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from <t>ANOVA</t> with Fisher’s least significant <t>difference</t> <t>(LSD)</t> post hoc test. *, p < 0.05; n.s., no statistical difference.
    Student’s T Test Or One Way Anova, Followed By Post Hoc Lsd Tests, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/student’s t test or one-way anova, followed by post hoc lsd tests/product/GraphPad Software Inc
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    Image Search Results


    Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from ANOVA with Fisher’s least significant difference (LSD) post hoc test. *, p < 0.05; n.s., no statistical difference.

    Journal: Cells

    Article Title: Pharmacological Blockade of the Adenosine A 2B Receptor Is Protective of Proteinuria in Diabetic Rats, through Affecting Focal Adhesion Kinase Activation and the Adhesion Dynamics of Podocytes

    doi: 10.3390/cells13100846

    Figure Lengend Snippet: Modulation of A 2B AR affects the adhesion and migration of podocytes. ( A ). Representative indirect immunofluorescence images of podocytes at a spreading of 60 min and treatments using adenosine (10 μM) or a combination of the A 2B AR antagonist MRS1754 (50 nM). The phalloidin staining of actin filaments is pseudo colored in green; Y397-phosphorylated FAK is in red; nuclear staining with DAPI is in blue. n = 3. Bar indicates 100 µm. ( B ). Representative wound healing assay images. Monolayer cultures of differentiated podocytes were exposed to adenosine and in combination with the A 2B AR antagonist MRS1754. Light microscopy images were captured at wounding and following 6 and 24 h. Bar indicates 300 µm. ( C ). Quantification of wound healing percentage from images of treated podocytes in ( B ). The graph represents means ± SEM. Five fields were analyzed by each condition. Four independent experiments were achieved. ( D ). Representative images of podocyte transmigration tests through Boyden’s chambers following treatments with adenosine and in combination with the A 2B AR antagonist MRS1754. Bar indicates 300 µm. ( E ). Quantitative analysis of transmigrated cells from experiments in ( D ). The graph depicts means ± SEM of the number of cells per field, 5 fields analyzed in each condition. n = 3. p values are shown on brackets in graphs ( C , D ) obtained from ANOVA with Fisher’s least significant difference (LSD) post hoc test. *, p < 0.05; n.s., no statistical difference.

    Article Snippet: Data were tabulated and analyzed by ANOVA with (LSD) post hoc test using GraphPad Prism 9.0.

    Techniques: Migration, Immunofluorescence, Staining, Wound Healing Assay, Light Microscopy, Transmigration Assay